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Investigations on Strophanthus.

From a paper by Mr. Wm. Elborne, published in Phar. Jour. and Trans., March 12, 1887, p. 743, we make the following extracts:

Strophanthus was introduced by Prof. Fraser, his researches having reference to the seeds of the Kombé arrow poison (See AMER. JOUR. PHAR., 1886, p. 405). From these seeds Fraser isolated a crystalline bitter glucoside, which he named strophanthin. From Strophanthus hispidus, De Cand., Hardy and Gallois subsequently isolated a crystalline bitter principle, neither of a glucosidal nor alkaloidal nature, but possessing all the toxic properties of Fraser's glucoside, which they termed strophantine (AMER. JOUR. PHAR., 1877, p. 402). The botany of the subject is by no means at present sufficiently clear to enable pharmacists to distinguish with precision the one from the other, and it appears to be questionable whether poisonous seeds, possessing undoubtedly the physiological activity described by Fraser, may be here and are not collected from species other than the two already experimented upon by the above gentlemen. Prof. Oliver has stated that the fruits entirely correspond in the two species; it is, however, generally accepted that the follicles yielding seeds with greenish-brown hairs, belong to the Kombé plant, whereas those yielding seeds with brown hairs, belong to the S. hispidus, and Prof. Oliver, after a more minute examination, referred the former to a distinct species which he named Strophanthus Kombé. This plant is described as follows by Dr. Kirk, Consul at Zanzibar:

[image:13620 align=left hspace=1]"The plant is a woody climber, growing in the forest both of the valley and the hills, and found at various places between the coast and the centre of the continent above the Victoria Falls and the Zambesi. The stem is several inches in diameter and rough outside. The plant climbs up the highest trees and hangs from one tree to another like a bush-vine. The flowers are of a pale yellow, and last for but a short time during the months preceding the first rains of the season. (Oct and Nov)."

The fruit is ripe in June. The natives separate the rough epicarp, and mesocarp, and dry the endocarp containing the seeds; hence the tawny appearance of the commercial follicles.

The method adopted by the natives in poisoning their arrows, is as follows: Before extracting the seed from the fruits, they dig a hole in the ground, so that they can bury the comose hair attached to the seed (for fear of its flying in their eyes), they then coarsely grind the seed, and mix it into a paste, which latter constitutes the poison with which the arrows are smeared. Game wounded by an arrow thus poisoned dies at once, seldom being able to move a hundred yards. The flesh is eaten without any evil effect accruing. The only precaution is to squeeze the sap out of a branch of the baobab tree into the wound made by the arrow, which is said to mitigate any evil effect that might result from the poison being more plentiful in the vicinity of the wound.

The drug examined by Mr. Elborne had been presented by Mr. T. Christy, and was collected in East Africa. Mr. E. M. Holmes found it to correspond with that from Lake Nyanza, which is referred to Str. Kombé. The seeds of Str. hispidus are chestnut-brown. The hairs on the seed are quite deciduous, and the comose appendages are white. One of the pods, 12 inches in length, weighed 14.069 gm., and yielded seeds 5.99 gm. (42 per cent.); comose hair 3.119 gm. (22 per cent.), and endocarp 4.96 gm. (35 percent.)

On submitting the seeds to analysis, petroleum ether dissolved 20.8 per cent. of a bright yellow oil, having a tinge of green, free from bitter taste, and in a few days depositing some colorless crystals which were fusible, and on ignition left no ash. Absolute ether took up 0.9 per cent. of chlorophyll and fat, and the extract was free from bitterness. The absolute alcohol extract, after treatment with charcoal, was obtained in transparent scales weighing 1.5 per cent.; it was soluble in water, imparted bitterness to 380.000 of water, did not react with alkaloid reagents, was not precipitated by lead acetate, and did not reduce Fehling's solution until it had been boiled with dilute sulphuric acid. Cold distilled water extracted 22.5 per cent. of extract, which when dissolved in little water and poured into a large quantity of a mixture of alcohol and ether, precipitated albuminous matters, and by evaporation of the filtrate yielded an additional quantity of 2.9 per cent. of bitter principle, identical with the preceding in appearance, behavior and physiological action. The matter not dissolved by the foregoing treatment weighed 54.3 per cent. According to L. Larmuth, the bitter principle on being dissolved in water will, in a few days, undergo some change, and become far more toxic than when recently prepared.

The comose hairs yielded to absolute alcohol 0.68 per cent. of brown extract, from which water dissolved a very small amount of slightly bitter matter, not acted upon by alkaloidal reagents. The resinous residue was insoluble in ether; its alcoholic solution had a bitter taste, and dropped into water produced a beautiful blue fluorescence. The aqueous extract of the hairs was free from bitterness.

The endocarp gave with absolute alcohol 1.3 per cent. of extract, yielding with water a slightly bitter solution, free from tannin and not precipitated by Mayer's solution.

The root, freed from the cortical portion, excited sneezing when powdered, and yielded to ether 0.7 per cent. of caoutchouc-like substance; to alcohol 1.1 per cent. of an intensely bitter substance giving the reaction for a glucoside; and to water 7.67 per cent. of a very bitter extract, which has not yet been examined.

H. Helbing (Phar. Jour. and Trans., March 12, 1887, p. 747), has found the quality of the Kombé seeds to vary considerably; the best are 15 to 25 mm. long, and 4 to 5 mm. broad, somewhat rounded at the base, narrowed at the apex and prolonged into the stalk of the hairy crown, somewhat twisted lengthwise, flattened, on one side with a much more prominent keel-like ridge than on the other, of a grayish-green to brown color, and covered with appressed silvery silky hairs; 100 seeds weigh about 62 grains. Another variety of seeds is similar to the preceding in shape, but densely covered with loose, longer, silky, white hairs like a fur; 100 seeds weigh about 57 grains. The least heavy of the commercial strophanthus, seeds have a dusky, dirty color, the kernel is not white, the hairs of the crown are dingy yellow, and 100 seeds weigh about 33 or 34 grains.

On drying the Kombé seed at 120° F. they lose upwards of 5 per cent. of moisture, and give with ether 32.45 per cent. of dark green fixed oil, sp. gr. .925, and becoming brownish-red when heated on the water bath. The white strophanthus seed yielded 23.33 per cent. oil, which was a little paler in color, but otherwise like the preceding.

Mr. Helbing likewise found that the seeds freed from oil cannot be completely deprived of bitterness by the use of rectified spirit sp. gr. .838. A tincture thus prepared is of a very pale color, has the sp. gr. .840, and a fluid ounce of it yields about 120 mgm. of residue on evaporation. Three commercial tinctures had nearly the same density, but yielded respectively 88, 124 and 180 mgm. of residue. Four other tinctures were probably made with a weaker alcohol, were of a green or yellow color, varied between .870 and .900 in density, and yielded from 170 to 242 mgm. of residue.

H. D. Rolleston, B. A., (Ph. Jour. and Trans., March 19,1887, p. 761), observed that the ethereal extract of the seed gave with distilled water a solution, which on being filtered from the oil, bad a bitter taste and the physiological effects of strophanthin. Similar results were obtained with the ethereal extract of strophanthus seeds prepared by different experimenters from white and green strophanthus seeds with absolute ether, showing that strophanthin is soluble in ether when the oil is present, and that the ethereal extract is not without value.

A. W. Gerrard, (Ph. Jour. and Trans., May 14, 1887, p. 923), obtained from strophanthus seeds by treatment with petroleum spirit, 31 per cent. of green fixed oil, and on subsequent treatment with 84 per cent. alcohol, 52 per cent. of extract. Using upon various samples of seeds successively petroleum benzin, ether and absolute alcohol, the latter yielded 5 per cent. of extract, or considerably more than bad been obtained by Elborne. The alcoholic extract may be obtained without the costly process of percolation with ether; on boiling the ground seeds with alcohol, and distilling and evaporating the tincture, about 5 per cent. of hard extract is obtained, from which the 31 per cent. of oil can be easily poured off, and adhering traces be washed away with very little ether. Elborne's results of the absence of an alkaloid, ineine, from the comose hairs are confirmed.

Strophanthin was prepared from the alcoholic extract, by dissolving it in water, filtering, adding excess of tannin, washing the gray precipitate with warm water, mixing with excess of lead acetate, drying the mixture, exhausting it with warm alcohol, removing lead by H2S, filtering and evaporating. Thus obtained, strophanthin is pale yellowish, amorphous, readily pulverizable, burns without residue, dissolves freely in water and alcohol, and is insoluble in absolute ether or chloroform. The watery solution, when shaken, gives much froth; warmed with silver nitrate the latter is reduced; tannin causes a white precipitate; on boiling with dilute sulphuric acid glucose is produced.

Helbing (ibid. p. 924), had observed that concentrated sulphuric acid dissolves strophanthin, changing, the color to dark green and finally dark reddish-brown. Minute traces of strophanthin may be detected by dissolving in a drop of water, adding a trace of solution of ferric chloride, and then a little concentrated sulphuric acid; a reddish-brown precipitate is formed which in the course of an hour or two turns emerald-green or a little darker-green, and this color remains unchanged for a long time.

Dr. F. F. Hanausek has published (Phar. Post, May 8, 1887, p. 301), a description of strophanthus seeds, from which the following abstract is made: Length of the seed 15 to 20 mm., width 4 mm., thickness about 1 mm., base rounded, apex attenuated to point which is prolonged into an awn almost 9 cm. long, the upper third of which is on all sides beset with delicate silky fragile hairs about 6 cm. in length. Seed yellowish-white, covered feltlike with soft silky hairs. The transverse section shows under the wrinkled testa a thin endosperm and two nearly plano convex cotyledons, the latter constituting the greater part of the seed. The section treated with potassa shows the testa colored golden-brown, the albumen colorless, and the cotyledons greenish or canary-yellow. Concentrated sulphuric acid colors the hairs and testa golden-brown, the albumen emerald-green, and the cotyledons yellow, changing successively to greenish, bronze-colored, coppery and finally almost blood-red. It appears from the reactions that the albumen contains principally fixed oil, and the embryo besides fat also strophantin.

A false strophanthus seed has been examined by Mr. E. M. Holmes (Phar. Jour. and Trans., May 7, 1887, p. 903), and shown to be the seed of Kicksia africana, Bentham, which grows on the Bagrooriver, at Fernando Po and at Bonny, in open low country, and is the only known African species. The seed is without awn, but is attached to the long hairy funiculus, which resembles a retrorsely hairy awn. On transverse section the cotyledons are seen to be folded or contortuplicate. Prof. Birch isolated from the seed a toxic principle, which is not a glucoside, but most likely an alkaloid. Prof. Kickx, after whom the genus is named, was director of the Botanic Garden at Ghent, and president of the Botanical Society of Belgium, and died March 20, 1887.

These seeds, as figured by T. Christy (New Commercial Plants, part 10), are pointed at both ends, somewhat bent, not hairy, but the retrorse hairs of the funiculus project beyond the apex of the seed.

Other strophanthus seeds are also figured and described by Christy. The seed of Str. hispidus, De Cand., are smaller than Kombé seeds, dark brown, short-hairy, the bare awn rather short. Str. dichotomus var. Marckii, De Cand., from Java, has the seed rounded, but narrowed at the base, dark-brown, flat, slightly bitter; bare awn short, brown, the hairy portion paler and the hairs long. The seed of an unknown species, resembles Kombé, but is larger, gray-green , has a much longer awn, and is very bitter. Another seed from the Gold Coast is pale-brown, scarcely bitter, the awn and awn-hairs rather short.

The seed of Str. Ledienii, Stein , (Gartenzeitung, 1887, p. 146; see also AMER. JOUR. PHAR., 1887, p. 269), is of the shape and size of a wheat grain, densely covered with silky yellowish-brown hairs, and at the apex provided with an awn, which is about 2 cm. long, and from its base beset with hairs, the total length of the comose appendage being about 5 cm.

J. M. M.


The American Journal of Pharmacy, Vol. 59, 1887, was edited by John M. Maisch.



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